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Recombinant bFGF (FGF2, basic fibroblast growth factor), human
| Catalogue Number | CYT-218 |
| Synonyms | Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b. |
| Introduction | Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. |
| Description | Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton. The FGF-b is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. Also available from Sf9 and HEK 293 cells. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 96.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino acid sequence | MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS. |
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2,000,000 Units/mg. |
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard. |
| References | 1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomas Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010 Link:http://neurooncology.oxfordjournals.org/content/12/8/790.full 2.Title:Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation Publication:Advance Online Publication March 4, 2008 doi: 10.1242/?jcs.019315 April 1, 2008 J Cell Sci 121, 1002-1013. Link:http://jcs.biologists.org/content/121/7/1002.full 3. Title:Embryonic Stem Cell-Derived Glial Precursors as a Vehicle for Sulfamidase Production in the MPS-IIIA Mouse Brain . Publication: Link:http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000008/art00007 4.Title:Adult Human Dental Pulp Stem Cells Differentiate Toward Functionally Active Neurons Under Appropriate Environmental Cues. Publication: Article first published online: 22 MAY 2008 DOI:10.1634/stemcells.2007-0979 Copyright © 2008 AlphaMed Press. Link:http://onlinelibrary.wiley.com/doi/10.1634/stemcells.2007-0979/full |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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